Separation science of macromolecules.

نویسنده

  • André M Striegel
چکیده

The end-use application of most macromolecules is determined not only by their chemical identity but also, and sometimes more importantly, by the distributions and sequences of key physical and physicochemical parameters. The ability to determine these distributions is gained through separation science, by enlisting the aid of a large number of separation methods in combination with spectroscopic, hydrodynamic, etc. methods of detection.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Using a Temperature Gradient against the Time in Polyacrylamide Gel Electrophoresis May Eliminate the Need for Stacking Gels

Background and Objectives: Making stacking gels for polyacrylamide gels in the laboratory by conventional methods is laborious and time consuming. Considering the role of temperature in polyacrylamide gels with respect to electrical resistance and viscosity, we assumed that decreasing the temperature would cause an increase in electrical resistance and viscosity.  Ultimately, a downward tempera...

متن کامل

Field-flow fractionation of proteins, polysaccharides, synthetic polymers, and supramolecular assemblies.

This review summarizes developments and applications of flow and thermal field-flow fractionation (FFF) in the areas of macromolecules and supramolecular assemblies. In the past 10 years, the use of these FFF techniques has extended beyond determining diffusion coefficients, hydrodynamic diameters, and molecular weights of standards. Complex samples as diverse as polysaccharides, prion particle...

متن کامل

Re-design of Downstream Processing Techniques for Nanoparticulate Bioproducts

There has been much interest generated in the recovery of nanoparticulate (nanoparticle) bioproducts(Second generation of biotechnological products) such as plasmid DNA and viruses as putative gene therapyvectors, macromolecular assemblies as drug delivery vehicles and virus-like particles as vaccine components.Such product must be manufactured in advanced stages of purity, ma...

متن کامل

Adsorption of charged macromolecules on mixed fluid membranes

The adsorption of charge rigid macromolecules, such as proteins from solution, on mixed (charged and neutral) lipid membranes is affected by several important factors. First, the mobile lipids in the membrane may rearrange, and demix locally to match the charge density of the apposed macromolecule, thus lowering the adsorption free energy. On the other hand, the (electrostatic) interaction betw...

متن کامل

Separation and characterization of natural and synthetic macromolecules.

www.lcgceurope.com A group of well-known chromatographers is involved in organizing this symposium, which is intended to bring together a variety of scientists, who share an interest in the separation and characterization of large molecules. Among the organizers are Robert Smits, a frequent guest editor of LC•GC Europe and the man behind the very successful symposium series on hyphenated techni...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Analytical and bioanalytical chemistry

دوره 399 4  شماره 

صفحات  -

تاریخ انتشار 2011